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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: Secreted Hsp90 Is a Novel Regulator of the Epithelial to Mesenchymal Transition (EMT) in Prostate Cancer
doi: 10.1074/jbc.M112.389015
Figure Lengend Snippet: Modest elevation of eHsp90 is sufficient to suppress E-cadherin function and promote cell motility. A, upper panel, ELISA analysis of secreted eHsp90 protein detected from conditioned media collected from parental ARCaPE cells stably transduced with control (lacZ) or V5-tagged eHsp90α lentivirus. Lower panel, immunoblot detection of total (endogenous and exogenous) eHsp90α, or V5 detection of transduced eHsp90 protein. B, immunoblot analysis of cell lysates from ARCaPE-LacZ or ARCaPE-eHsp90 confirmed consistent levels of intracellular eHsp90α (IC Hsp90). Indicated analysis of E- and N-cadherin and ERK activity. C, representative morphology of the indicated ARCaPE cells. Analysis of cell motility of ARCaPE-eHsp90 either untreated or treated with NPGA. D, effect of NPGA upon E-cadherin expression in ARCaPE-eHsp90. E, analysis of E-cadherin localization in ARCaPE-LacZ and ARCaPE-eHsp90 untreated cells, or treated for the indicated times with NPGA. F, membrane localization of ZO1 in ARCaPE-LacZ and ARCaPE-eHsp90 untreated cells, or treated with NPGA for 3 days. Asterisks (*) indicate significance of p value ≤0.05. Scale bar is 50 μm.
Article Snippet:
Techniques: Enzyme-linked Immunosorbent Assay, Stable Transfection, Transduction, Western Blot, Activity Assay, Expressing
Journal: iScience
Article Title: tANCHOR-cell-based assay for monitoring of SARS-CoV-2 neutralizing antibodies rapidly adaptive to various receptor-binding domains
doi: 10.1016/j.isci.2024.109123
Figure Lengend Snippet:
Article Snippet:
Techniques: Virus, Recombinant, Saline, Staining, Isolation, Protease Inhibitor, Software, Cell Culture, Enzyme-linked Immunosorbent Assay